Saturday, November 19, 2011

What nutrients would be present in an agar medium used to grow microorganisms?

What nutrients would be present in an agar medium used to grow microorganisms?

What nutrients would be present in an agar medium used to grow microorganisms?
Plenty 'o riboflavin!
Reply:The most common growth media for microorganisms are nutrient broths (liquid nutrient medium) or Lysogeny broth (LB medium).





Nutrient media:-





Undefined media (also known as basal or complex media) is an undefined media that contains:





* a carbon source such as glucose for bacterial growth


* water


* various salts need for bacterial growth


* a source of amino acids and nitrogen (e.g., beef, yeast extract).
Reply:Usually a carbon source, nitrogen source and a number of amino acids depending on the microorganism in question...


The aforementioned LB (Luria-Bertani) medium is a very simple medium containing only yeast extract, peptone and sodium chloride. Yeast extract contains a range of nutrient sources on its own already... Well...and agar of course, although that's just a gelling agent, and can't be used as a nutrient source by most microorganisms.
Reply:It depends what you intend to grow and what you are trying to acheive.....





The basic agar nutrient is called 'Broth' and is a meat extract.





For basic experiments some people cook and puree lambs liver and add that to the agar.





There are lots and lots of various mediums. If you intend to study one particular organism, let me know and I will tell you the best options.

Teeth Cleaning

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